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Chen, Wei-Yi


  • Assistant Professor
  • Education: PhD. National Defense Medical College (Institute of Life Science) ; Postdoc. Lab of Biochemistry and Molecular Biology, Rockefeller University, USA
  • Office: R711, 7F, Tradition Medicine Building
  • Phone: 886-2-2826-7328

Research--Transcriptional Regulatory Mechanism of B cell Lymphopoiesis and Leukemogenesis

  Antibody producing B cells arise from hematopoietic stem cells through a series of developmental steps that involve a hierarchical transcription factor network. Whereas much is known about their direct genetic targets, little is known about their actual mechanism of action through interactions with various coactivators that act on chromatin structure or on the general transcription machinery. In addition, genetic mutations of the involved transcription factors result in loss-of-function or gain-of-function frequently lead to B cell deficiency or malignancy.

  Our goal is to uncover the mechanisms by which transcriptional activators/coactivators (including various histone modifiers/remodelers) control the development and leukemogenesis of B-lymphocytes, as well as searching therapeutic targets/approaches for B-cells associated leukemia/lymphoma.

  On-going studies will employ various state-of-the-art biomedical technologies that include:

1. biochemically isolating recombinant proteins/protein complexes from bacteria/insect/mammalian cell cultures for cell-free assays;

2. genome-widely profiling (ChIP-seq and RNA-seq) the transcription-related factors (DNA-binding proteins and transcriptional coactivators) and corresponding histone modifications in normal or malignant cells; 

3. developing the peptidomimetics for potential therapeutic to leukemia/lymphoma treatment. 


  • Jiang BH, Chen W-Y, Li HY, Chien Y, Chang WC, Hsieh PC, Ping Wu P, Chen CY, Song HY, Chien CS, Sung YJ, and Chiou SH. CHD1L regulated PARP1-driven Pluripotency and Chromatin Remodeling during the Early-stage Cell Reprogramming. Stem Cells 33, 2961-2972 (2015).


  • Geng H, Hurtz C, Lenz KB, Chen Z, Baumjohann D, Thompson S, Goloviznina N, Chen W-Y, Huan J, LaTocha D, Ballabio E, Xiao G, Lee JW, Deucher A, Qi Z, Park E, Huang C, Nahar R, Kweon SM, Shojaee S, Chan LN, Yu J, Kornblau SM, Bijll JJ,Ye BH, Ansel M, Paietta E, Melnick A, Hunger SP,  Kurre P, Tyner JW, Loh ML, Roeder RG, Druker BJ, Burger JA, Milne TA, Chang BH, and Müschen M. Self-enforcing feedback activation between BCL6 and pre-B cell receptor signaling defines a distinct subtype of acute lymphoblastic leukemia. Cancer Cell 27, 409-425 (2015)


  • Cheng KW, Cheng SC, Chen W-Y, Lin MH, Chuang SJ, Cheng IH, Sun CY, and Chou CY. Thiopurine analogues and mycophenolic acid synergistically inhibit the papain-like protease of Middle East respiratory syndrome coronavirus. Antiviral Research 115, 9-16 (2015)


  • Chen C-H, Chen W-Y, Lin S-F, and Wong RJ. Epithelial-mesenchymal transition enhances response to oncolytic herpes viral therapy through nectin-1. Human Gene Therapy 25, 539-551 (2014)


  • Chen W-Y, Zhang J, Geng H, Du Z, Nakadai T, and Roeder RG. A TAF4 coactivator function for E proteins that involves enhanced TFIID binding. Genes and Development 27, 1596-1609 (2013).


  • Tang Z, Chen W-Y, Shimada M, Nguyen UTT, Kim J, Sun XJ, Sengoku T, McGinty RK, Fernandez JP, Muir TW, and Roeder RG. SET1 and p300 synergy, through coupled histone modifications, in transcriptional activation by p53. Cell 154, 297-310 (2013).


  • Sun XJ, Wang Z, Wang L, Jiang Y, Kost N, Soong TD, Chen W-Y, Tang Z, Nakadai T, Elemento O, Fischle W, Melnick A, Patel DJ, Nimer SD, and Roeder RG. A stable transcription factor complex nucleated by oligomeric AML1-ETO controls leukemogenesis. Nature 500, 93-97 (2013).


  • Cai L, Rothbart SB, Lu R, Xu B, Chen W-Y, Tripathy A, Rockowitz S, Zheng D, Patel DJ, Allis CD, Strahl BD, Song J, and Wang GG. An H3K36 methylation engaging Tudor motif of polycomb-like proteins mediates PRC2 complex targeting. Molecular Cell 49, 571-582 (2013).


  • Geng H, Brennan S, Milne TA, Chen W-Y, Li Y, Hurtz C, Kweon SM, Zickl L, Shojaee S, Neuberg D, Huang C, Biswas D, Xin Y, Racevskis J, Ketterling RP, Luger SM, Lazarus H, Tallman MS, Rowe JM, Litzow MR, Guzman ML, Allis CD, Roeder RG, Muschen M, Paietta E, Elemento O, Melnick AM. Integrative Epigenomic Analysis Identifies Biomarkers and Therapeutic Targets in Adult B-Acute Lymphoblastic Leukemia. Cancer Discovery 2, 1004-1023 (2012).


  • Wang C-Y, Kao Y-H, Lai P-Y, Chen W-Y, and Chung B-c. Steroidogenic Factor-1 (NR5A1) maintains centrosome homeostasis in steroidogenic cells by restricting centrosomal DNA-PK activation. Mol Cell Biol 33, 476-484 (2013).


  • Wang C-Y, Chen W-Y, Lai P-Y, and Chung B-c. Distinct functions of steroidogenic factor-1 (NR5A1) in the nucleus and the centrosome. Mol Cell Endocrinol 371, 148-153 (2013).


  • Lai P-Y, Wang C-Y, Chen W-Y, Kao Y-H, Tsai H-M, Tachibana T, Chang WC, and Chung B-c. Steroidogenic Factor 1 (NR5A1) resides in centrosomes and maintains genomic stability by controlling centrosome homeostasis. Cell Death and Differentiation 18, 1836-1844 (2011).


  • Jiang H, Shukla A, Wang X, Chen W-Y, Bernstein BE, and Roeder RG. Role for Dpy-30 in ES cell-fate specification by regulation of H3K4 methylation within bivalent domains. Cell 144, 513-525 (2011).


  • Chen W-Y, Weng J-H, Huang C-C, and Chung B-c. Histone deacetylase inhibitors reduce steroidogenesis through SCF-mediated ubiquitination and degradation of steroidogenic factor 1 (NR5A1). Mol Cell Biol 27, 7284-7290 (2007).


  • Lee LS, Shu WJ, Wu CM, Hsieh CH, Chen SM, Hu CJ, Chen W-Y, and Chung B-c. A novel compound heterozygous mutation of K494_V495 deletion plus R496L and D487_F489 deletion in extreme C-terminus of cytochrome P450c17 causes 17alpha-hydroxylase deficiency. Mol Cell Endocrinol 249, 16-20 (2006).


  • Chen W-Y, Juan L-J, and Chung B-c. SF-1 (nuclear receptor 5A1) activity is activated by cyclic AMP via p300-mediated recruitment to active foci, acetylation, and increased DNA binding. Mol Cell Biol 25, 10442-10453 (2005).


  • Chen W-Y, Lee WC, Hsu NC, Huang F, and Chung B-c. SUMO modification of repression domains modulates function of nuclear receptor 5A1 (steroidogenic factor-1). J Biol Chem 279, 38730-38735 (2004).


  • Chen W-Y, Yang JG, and Li PS Effect of dexamethasone on the expression of p34(cdc2) and cyclin B1 in pig oocytes in vitro. Mol Reprod Dev 56, 74-79 (2000).


  • Yang JG, Chen W-Y, and Li PS. Effects of glucocorticoids on maturation of pig oocytes and their subsequent fertilizing capacity in vitro. Biol Reprod 60, 929-936 (1999).


  • Chen W-Y, Yang JG, Huang SH, and Li PS. Effects of cyclophosphamide on maturation and subsequent fertilizing capacity of pig oocytes in vitro. Chin J Physiol 41, 75-83 (1998).


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