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Tsay, Yeou-Guang
  • Education: PhD (Biochemistry), Michigan State University
  • Office: R645, 6F, Library and Information Building
  • Phone: 886-2-28267119
  • Email: This e-mail address is being protected from spambots. You need JavaScript enabled to view it
  • Personal Web Site
  • Research--The functional roles of posttranslational modifications and protein quaternary structures (or protein complexes)
  • The primary interest of our laboratory is to understand the functional roles of two classes of sophisticated protein structures, namely posttranslational modifications and quaternary structures (or protein complexes). The metabolism of these protein structures are long believed to be the fundamental mechanisms that modulate protein functions in many important biological processes. However, for most proteins, how the protein activities are governed by their protein modification or/and complex assembly remains poorly understood. In order to facilitate the researches on delineations of the relationships between functions and these protein structures, our laboratory is dedicated to acquisition, verification and organization of the information about posttranslational modifications and quaternary structures. In order to accomplish this, we have developed several novel mass-spectrometry-based proteomics platforms through integration of various techniques in protein chemistry and bioinformatics. We are now applying these new technologies to acquire the basic information essential for identification and verification of the protein structures that are keys to major physiological and pathophysiological biological processes.
  • The ongoing projects in our laboratory include: (1) To constantly improve the throughput of our comprehensive posttranslational modification mapping procedure for identification of protein modifications present in the proteome; (2) To optimize the specificity and completeness of immobilized metal ion affinity chromatography (IMAC) for effective identification of protein phosphorylation sites in the entire proteome; (3) To develop new techniques for determination of monoisotopic masses of regular proteins for rapid sequence verification of the designed proteins and for initial screening of protein variants in regular biological samples. (4) To develop a protein complex mapping procedure for collection and organization of basic information on protein primary and quaternary structures. (5) To develop proteomic assays for identification of differentially expressed protein complexes between two or more proteomes. (6) To develop proteomic assays specifically for cataloging the protein complex structures present in the human plasma proteome (7) To establish posttranslational modification databases of human proteomes via our comprehensive posttranslational modification mapping procedure for biomarker discovery (8) To establish protein complex databases for a variety of proteomes, such as human plasma, colon cancer tissue, liver cancer tissue, Arabidopsis seedling, mouse liver tissue, mouse brain tissue, mouse embryonic stem cell, human mesenchymal stem cell and others. (9) To use human albumins as the prime example for understanding how differential arrangements of protein modifications may diversify the quaternary structures of a protein with well defined amino acid sequence.
  • Publications
    • Touré A, Mzali R, Liot C, Seguin L, Morin L, Crouin C, Chen-Yang I, Tsay YG, Dorseuil O, Gacon G, Bertoglio J. (2008) Phosphoregulation of MgcRacGAP in mitosis involves Aurora B and Cdk1 protein kinases and the PP2A phosphatase. FEBS Lett. In Press.

    • Wu TF, Ku WL, Tsay YG. (2007) Proteome-based diagnostics and prognosis of bladder transitional cell carcinoma. Expert Rev Proteomics. 4(5):639-47.

    • Huang C, Chang SC, Yu IC, Tsay YG, Chang MF. (2007) Large hepatitis delta antigen is a novel clathrin adaptor-like protein. J Virol. 81(11):5985-94.

    • Huang CH, Tsai HH, Tsay YG, Chien YN, Wang SL, Cheng MY, Ke CH, Chen CW. (2007) The telomere system of the Streptomyces linear plasmid SCP1 represents a novel class. Mol Microbiol. 63(6):1710-8.

    • Chang YW, Chen SC, Cheng EC, Ko YP, Lin YC, Kao YR, Tsay YG, Yang PC, Wu CW, Roffler SR. (2005) CD13 (aminopeptidase N) can associate with tumor-associated antigen L6 and enhance the motility of human lung cancer cells. Int J Cancer. 116(2):243-52.

    • Tsay YG, Cheng CC, Hu ST (2005) Identification of the -1 translational frameshift sites using liquid chromatography-tandem mass spectrometry (2005) Anal. Biochem. 339(1):83-93

    • Lin SK, Chang MC, Tsay YG, Lur SH (2005) Proteomic analysis of the expression of proteins related to rice quality during caryopsis development and the effect of high temperature on expression. Proteomics. 5(8):2140-2156.

    • Lai LP, Lin JL, Lin CS, Yeh HM, Tsay YG, Lee CF, Lee HH, Chang ZF, Hwang JJ, Su MJ, Tseng YZ, Huang SK. (2004) Functional genomic study on atrial fibrillation using cDNA microarray and two-dimensional protein electrophoresis techniques and identification of the myosin regulatory light chain isoform reprogramming in atrial fibrillation. J Cardiovasc Electrophysiol. 15(2):214-23.

    • Mu JJ, Tsay YG, Juan LJ, Fu TF, Huang WH, Chen DS, Chen PJ. (2004) The small delta antigen of hepatitis delta virus is an acetylated protein and acetylation of lysine 72 may influence its cellular localization and viral RNA synthesis. Virology. 319(1):60-70.

    • Hsu RB, Tsay YG, Wang SS, Chu SH. (2003) Management of aortic aneurysm infected with Salmonella. Br J Surg. 90(9):1080-4.

    • Hsu RB, Tsay YG, Lee CM, Chen RJ, Wang SS, Chu SH. (2003) Soluble inflammatory markers in coronary sinus and peripheral blood of heart transplant recipients. Clin Transplant. 17(4):331-7.

    • Wang YH, Tsay YG, Tan BC, Lo WY, Lee SC. (2003) Identification and characterization of a novel p300-mediated p53 acetylation site, lysine 305. J Biol Chem. 278(28):25568-76.

    • Hsu RB, Tsay YG, Chen RJ, Chu SH. (2003) Risk factors for primary bacteremia and endovascular infection in patients without acquired immunodeficiency syndrome who have nontyphoid salmonellosis. Clin Infect Dis. 36(7):829-34.

    • Hsu RB, Tsay YG, Wang SS, Chu SH. (2002) Surgical treatment for primary infected aneurysm of the descending thoracic aorta, abdominal aorta, and iliac arteries. J Vasc Surg. 2002 Oct;36(4):746-50.

    • Lin WC, Shen BJ, Tsay YG, Yen HC, Lee SC, Chang CJ. (2002) Transcriptional activation of C/EBPbeta gene by c-Jun and ATF2. DNA Cell Biol. 21(8):551-60.

    • Chen HC, Lin WC, Tsay YG, Lee SC, Chang CJ. (2002) An RNA helicase, DDX1, interacting with poly(A) RNA and heterogeneous nuclear ribonucleoprotein K. J Biol Chem. 277(43):40403-9.

    • Chiu CM, Tsay YG, Chang CJ, Lee SC. (2002) Nopp140 is a mediator of the protein kinase A signaling pathway that activates the acute phase response alpha1-acid glycoprotein gene. J Biol Chem. 277(42):39102-11.

    • Chen CW, Tsay YG, Wu HL, Lee CH, Chen DS, Chen PJ. (2002) The double-stranded RNA-activated kinase, PKR, can phosphorylate hepatitis D virus small delta antigen at functional serine and threonine residues. J Biol Chem. 277(36):33058-67.

    • Yang CC, Huang CH, Li CY, Tsay YG, Lee SC, Chen CW. (2002) The terminal proteins of linear Streptomyces chromosomes and plasmids: a novel class of replication priming proteins. Mol Microbiol. 43(2):297-305.

    • Chia JS, Chang LY, Shun CT, Chang YY, Tsay YG, Chen JY. (2001) A 60-kilodalton immunodominant glycoprotein is essential for cell wall integrity and the maintenance of cell shape in Streptococcus mutans. Infect Immun. 69(11):6987-98. Erratum in: Infect Immun 70(3):1665.

    • Tsay YG, Wang YH, Chiu CM, Shen BJ, Lee SC. (2000) A strategy for identification and quantitation of phosphopeptides by liquid chromatography/tandem mass spectrometry.Anal Biochem. 287(1):55-64.

    • Tsay YG, Lin NY, Voss PG, Patterson RJ, Wang JL. (1999) Export of galectin-3 from nuclei of digitonin-permeabilized mouse 3T3 fibroblasts. Exp Cell Res. 252(2):250-61.

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