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Chang, Zee-Fen
  • Professor
  • Education: PhD. Department of Biochemistry, Rutgers University
  • Office: R923, 9F, Library and Information Building
  • Phone: 886-2-28267938
  • This e-mail address is being protected from spambots. You need JavaScript enabled to view it
Research--Nucleotide metabolism, cytoskeleton regulation and gene expression

1. Nucleotide metabolism in DNA damage

The molecular mechanism regulating dTTP synthesis is coordinated with DNA damage signal for repair. We focus on two research aspects: (i) the mechanism for DNA damage site-specific production of dTTP, (ii) design of small molecules targeting dTTP formation for specific intervention of DNA repair in malignant tumor cells.


2. Assessment of the Rho-ROCK pathway in differentiation and stress response

RhoA/ROCK-mediated contractility is critical for many cellular processes. Currently, we are interested in understanding how RhoA-ROCK pathway regulates gene expression in osteoblast committment of mesenchymal stem cell and centrosomal overduplication in response to DNA damage.


Ning Tsao, Ming-Hsiang Lee, Wei Zhang, Yung-Chi Cheng, and Zee-Fen Chang*. The contribution of CMP kinase to the efficiency of DNA repair. Cell Cycle 14:3, 1--10; December 1, 2014


Ming-Hsiang Lee, Liya Wang and Zee-Fen Chang*. The contribution of mitochondrial thymidylate synthesis in preventing the nuclear genome stress. Nucleic Acids Research, 2014,1-13 doi:10.1093/nar/gku152


Tien SC and Chang ZF*. Oncogenic Shp2 disturbs microtubule regulation to cause HDAC6-dependent ERK hyperactivation. Oncogene. 2013 Jun 17. doi: 10.1038/onc.2013.241. [Epub ahead of print]


Pan YR, Cho KH, Lee HH, Chang ZF*, Chen HC. Protein tyrosine phosphatase SHP2 suppresses podosome rosette formation in Src-transformed fibroblasts. J Cell Sci. 2013 Jan 15;126(Pt 2):657-66. doi: 10.1242/jcs.116624.


Hsiang-Hao Chuang, Shao-Wei Liang, Zee-Fen Chang* and Hsiao-Hui Lee*. Ser1333 phosphorylation indicates ROCKI activation. Journal of Biomedical Science 2013, 20:83


Chuang HH, Yang CH, Tsay YG, Hsu CY, Tseng LM, Chang ZF*, Lee HH*. ROCKII serine 1366 phosphorylation reflects the activation status. Biochemical Journal. 443: p145-151, 2012. (*: corresponding authors)


Hu, CM, Yeh, MT, Tsao, N, Chen, CW, Gao, QZ, Chang, CY, Lee, MH, Fang, JM, Sheu, SY, Lin, CJ, Tseng, MC, Chen, YJ, Chang, ZF*. Tumor Cells Require Thymidylate Kinase to Prevent dUTP Incorporation during DNA Repair. Cancer cell, 22(1) 36 - 50, 2012.


Lee HH, Tien SC, Jou TS, Chang YC, Jhong JG, and Chang ZF*. c-Src-dependent phosphorylation of ROCK participates in regulation of focal adhesion dynamics. J Cell Science. 123:3368-3377, 2010.


Chen YL, Eriksson S, Chang ZF*. The regulation and functional contribution of thymidine kinase 1 in repair of DNA damage. J Biol Chem. 285:27327-27335, 2010.


Hu CM, Chang ZF*. A bioluminescent method for measuring thymidylate kinase activity suitable for high-throughput screening of inhibitor. Anal Biochem. 2009 Dec 5. doi:10.1016/j.ab.2009.12.001


Chang YC, Tien SC, Tien HF, Zhang H, Bokoch GM and Chang ZF*. p210Bcr_Abl desensitizes Cdc42 GTPase signaling for SDF-1a-directed migration in chronic myeloid leukemia cells. Oncogene. 28:4105-15, 2009.


Lee HH and Chang ZF*. Regulation of RhoA-dependent ROCK activation by Shp2. J. Cell Biol. 181:999-1012, 2008.


Chen YL, Lin DW, and Chang ZF*. Identification of a Putative Human Mitochondrial Thymidine Monophosphate Kinase Associated with Monocytic/Macrophage Terminal Differentiation. Genes to Cells. 13: 679-89, 2008.


Hu CM and Chang ZF*. Synthetic lethality by lentiviral short hairpin RNA silencing of thymidylate kinase and doxorubicin in colon cancer cells regardless of the p53 status. Cancer Res. 68:2831-40, 2008.


Chang YC, Nalbant P, Birkenfeld J, Chang ZF*, Bokoch, GM. GEF-H1 couples nocodazole-induced microtubule disassembly to Cell Contractility via RhoA. Mol Biol Cell. 19:2147-53, 2008.


Chou WL, Chuang LM, Chou CC, Wang AHJ, Lawson JA, FitzGerald, GA and Chang ZF*. Identification of a novel prostaglandin reductase reveals the involvement of prostaglandin E2 catabolism in regulation of peroxisome proliferator-activated receptor gamma activation. J Biol Chem. 282:18162-72, 2007.


Kuo YY, and Chang ZF*. GATA-1 and Gfi-1B interplay to regulate Bcl-xL transcription. Mol Cell Biol. 27:4261-72, 2007.


Ke PY, Hu CM., Chang YC, and Chang ZF*. Hiding human thymidine kinase 1 from APC/C-mediated destruction by thymidine binding. FASEB J. 21:2176-84, 2007.


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